Journal article
AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana
X Jin, Z Lv, J Gao, R Zhang, T Zheng, P Yin, D Li, L Peng, X Cao, Y Qin, S Persson, B Zheng, P Chen
Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2019
DOI: 10.1093/nar/gky1205
Abstract
Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to the nucleus and its function for m1G and m1I methylation was confirmed by mutant analysis, yeast complementation, m1G nucleoside level on single tRNA, and tRNA in vitro methylation. Arabidopsis attrm5a mutants were dwarfed and had short filaments, which led to reduced seed setting. Proteomics data indicated differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, ox..
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Grants
Awarded by Fundamental Research Funds of China West Normal University
Funding Acknowledgements
Natural Science Foundation of China [31370604 to B.Z., 31100268 to P.C.]; Natural Science Foundation of Hubei Province [2016CFB438 to P.C.]; Fundamental Research Funds for the Central Universities, China [2662015PY168 to P.C.]; S.P. was funded by an ARC FT grant [FT160100218] and acknowledges an UoM IRRTF (RNC) grant. Y.Q. was funded by the National Key R&D Program of China(2018YFA0106900); Strategic Priority Research Programs (Category A) of the Chinese Academy of Sciences XDA12010313, and Key Research Program of Frontier Sciences, CAS, (QYZDB-SSW-SMC028). Funding for open access charge: National Natural Science Foundation of China [31370604 to B.Z].